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Publications

2024

  • Singh, A., Jaiswal, S. K., Prakash, R., Mihara, H., and Prakash, N. T. (2024) Selenium Nanoparticles Synthesized and Stabilized by Fungal Extract Exhibit Enhanced Bioactivity J Clust Sci 10.1007/s10876-024-02600-5

  • Ochi, A., and Mihara, H. (2024) Effects of genetic disruption in thioredoxin and glutathione systems on selenium nanoparticle formation, selenite sensitivity, and selenoprotein biosynthesis in Escherichia coli Metallomics Research 4, reg14-reg20 https://doi.org/10.11299/metallomicsresearch.MR202401

2023

  • Takahashi, K., Ochi, A., Mihara, H., and Ogra, Y. (2023) Comparison of Nutritional Availability of Biogenic Selenium Nanoparticles and Chemically Synthesized Selenium Nanoparticles Biol. Trace Elem. Res. 201, 4861-4869 10.1007/s12011-023-03567-6

  • 青野陸, 井上真男, and 三原久明 (2023) 必須微量元素セレンの微生物による多彩な代謝 ファルマシア 59, 185-189 10.14894/faruawpsj.59.3_185

  • 越智杏奈, 井上真男, 青野陸, and 三原久明 (2023) 生態系においてセレン循環を駆動する微生物 金属 93, 162-168,

2022

  • Imai, T., Tobe, R., Honda, K., Tanaka, M., Kawamoto, J., and Mihara, H. (2022) Group II truncated haemoglobin YjbI prevents reactive oxygen species-induced protein aggregation in Bacillus subtilis eLife 11, e70467 10.7554/eLife.70467

  • Takeshi Imai, Ryuta Tobe, Koji Honda, Mai Tanaka, Jun Kawamoto, Hisaaki Mihara (2022) Group II truncated haemoglobin YjbI prevents reactive oxygen species-induced protein aggregation in Bacillus subtilis eLife 11:e70467  https://doi.org/10.7554/eLife.70467

2021

  • Shimizu, A., Tobe, R., Aono, R., Inoue, M., Hagita, S., Kiriyama, K., Toyotake, Y., Ogawa, T., Kurihara, T., Goto, K., Prakash, N. T., and Mihara, H. (2021) Initial step of selenite reduction via thioredoxin for bacterial selenoprotein biosynthesis. Int J Mol Sci 22, 10965

  • Yoshioka, H., Ishida, T., and Mihara, H. (2021) Overexpression and characterization of Escherichia coli dihydropyrimidine dehydrogenase: a four iron-sulphur cluster containing flavoprotein. J Biochem 170, 511-520

  • Inoue, M., Hirose, Y., Tobe, R., Saito, S., Aono, R., Prakash, N. T., and Mihara, H. (2021) Complete genome sequence of Pseudomonas stutzeri strain F2a, isolated from seleniferous soil. Microbiol. Resour. Announc. 10, e0063121

  • 三原久明. (2021) 細菌によるカルコゲン代謝. in 生命金属ダイナミクス～生体内における金属の挙動と制御～ (城宜嗣、津本浩平 ed.), エヌ・ティー・エス. pp 93-97

2020

  • Hirose, Y., Kumsab, J., Tobe, R., and Mihara, H. (2020) Complete genome sequence of an acetic acid bacterium, Acetobacter aceti JCM20276. Microbiol Resour Announc 9, e00962-00920. doi: 10.1128/MRA.00962-20.

  • Kumsab, J., Tobe, R., Kurihara, T., Hirose, Y., Omori, T., and Mihara, H. (2020) Characterization of a novel class of glyoxylate reductase belonging to the beta-hydroxyacid dehydrogenase family in Acetobacter aceti. Biosci. Biotechnol. Biochem. 84, 2303-2310. doi: 10.1080/09168451.2020.1797470.

  • Kaewkod, T., Tobe, R., Tragoolpua, Y., and Mihara, H. (2020) Medicinal plant extracts protect epithelial cells from infection and DNA damage caused by colibactin-producing Escherichia coli, and inhibit the growth of bacteria. J. Appl. Microbiol.130, 769-785. doi: 10.1111/jam.14817.

  • Jin, Y., Chung, Y. W., Jung, M. K., Lee, J. H., Ko, K. Y., Jang, J. K., Ham, M., Kang, H., Pack, C. G., Mihara, H., and Kim, I. Y. (2020) Apolipoprotein E-mediated regulation of selenoprotein P transportation via exosomes. Cell. Mol. Life Sci. 77, 2367-2386. doi: 10.1007/s00018-019-03287-y.

  • 三原久明. (2020) ジオマイクロバイオロジー. in 遺伝子・細胞から見た応用微生物学 (阪井康能, 竹川薫, 橋本渉, and 片山高嶺 eds.), 朝倉書店. pp 173

  • 三原久明. (2020) 補因子、補酵素の生合成と相分離. in 相分離生物学の全貌 (白木賢太郎 ed.), 東京化学同人. pp 53-57

2019

  • Jahan, M. I., Juengwiwattanakitti, P., Izu, Y., Tobe, R., Imai, T., and Mihara, H. (2019) Selenite uptake by outer membrane porin ExtI and its involvement in the subcellular localization of rhodanese-like lipoprotein ExtH in Geobacter sulfurreducens. Biochem. Biophys. Res. Commun. 516, 474-479.

  • Tamura, T., Nguyen, D. T., Fujita, D., Tobe, R., Mihara, H., and Taguchi, K. (2019) Effect of ΔiscS deficiency on current generation of E. coli in Microbial Fuel Cells. Adv. in Nat. Appl. Sci. 13, 15-21

  • Sato, K., Nguyen, D. T., Fujita, D., Tobe, R., Mihara, H., and Taguchi, K. (2019) High Performance Activated Carbon Paper-Based electrode for Microbial Fuel Cells. Adv. in Nat. Appl. Sci. 13, 7-14.

2018

  • Tobe R, Mihara H. (2018) "Delivery of selenium to selenophosphate synthetase for selenoprotein biosynthesis" Biochim Biophys Acta. 1862, 2433-2440.

  • Jahan MI, Tobe R, Mihara H. (2018) " Characterization of a novel porin-like protein, ExtI, from Geobacter sulfurreducens and its implication in the reduction of selenite and tellurite" Int. J. Mol. Sci. 19/3, E809.

  • 老川典夫、大島敏久、保川清、三原久明、宮原郁子 (2018)『エッセンシャル タンパク質工学』   講談社 .

  • 三原久明. (2018) 『毒か？栄養素か？　必須微量元素セレンの生物学』海洋科学研究 31, 95-100

2017

  • Tobe R, Koga H, Tani Y, Tajima H, Tsuji A, Mihara H. (2017) "Identification of selenomethionine, selenocysteine, and Se-methylselenocysteine in the selenium-enriched common ice plant" Biomed Res Trace Elements. 28, 135-142.

  • Staicu LC, Oremland RS, Tobe R, Mihara H. (2017) "Bacteria versus Selenium: A View from the Inside Out." Selenium in plants. Springer. Chapter 6.

  • Tobe R and Mihara H. (2017) 『鉄硫黄クラスターおよびセレンタンパク質生合成とその分子機構』硫酸と工業. 70, 23-28.

2016

  • Mihara H, Tobe R, Esaki N. (2016) "Mechanism, structure, and biological role of selenocysteine lyase" Selenium. Springer. Chapter 10, 113-123.

  • Carlson BA, Lee BJ, Tsuji PA, Tobe R, Park JM, Schweizer U, Gladyshev VN, Hatfield DL. (2016) "Selenocysteine tRNA[Ser]Sec: from nonsense suppressor tRNA to the quintessential constituent in selenoprotein biosynthesis" Selenium. Springer. Chapter 1, 3-12.

  • Hatfield DL, Carlson BA, Tsuji PA, Tobe R, Gladyshev VN. (2016) "Selenium and Cancer" Molecular, Genetic, and Nutritional Aspects of Major and Trace Minerals. Academic Press. Chapter 38, 463-473.

  • Tobe R, Tani Y, Kataoka M, Yamashita Y, Mihara H. (2016) "Relationship between the glycosphingolipids and phospholipids synthesis and the mycelial growth in Neurospora crassa" Trace Nutrients Research. 33, 13-20.

  • Tobe R. (2016) 『セレンも世界を救う』バイオミディア．94, 426.

  • Kamada S, Okugochi T, Asano K, Tobe R, Mihara H, Nemoto M, Inagaki K, Tamura T. (2016) "A non-radioactive assay for selenophosphate synthetase activity using recombinant pyruvate pyrophosphate dikinase from Thermus thermophilus HB8" Biosci Biotechnol Biochem. 80, 1970-1972.

  • Carlson BA, Tobe R, Yefremova E, Tsuji PA, Hoffmann VJ, Schweizer U, Gladyshev VN, Hatfield DL, Conrad M. (2016) "Glutathione peroxidase 4 and vitamin E cooperatively prevent hepatocellular degeneration" Redox Biol. 26, 22-31.

  • Tobe R, Carlson BA, Huh JH, Castro NP, Xu XM, Tsuji PA, Lee SG, Bang J, Na JW, Kong YY, Beaglehole D, Southon E, Seifried H, Tessarollo L, Salomon DS, Schweizer U, Gladyshev VN, Hatfield DL, Lee BJ. (2016) "Selenophosphate synthetase 1 is an essentialprotein with roles in regulation of redox homeostasis in mammals" Biochem J. 473, 2141-2154.

  • Schoenmakers E, Carlson BA, Agostini M, Moran C, Rajanayagam O, Bochukova E, Tobe R, Peat R, Gevers E, Muntoni F, Guicheney P, Schoenmakers N, Lyons G, Hatfield DL, Chatterjee K. (2016) "Mutation in human selenocysteine transfer RNA selectively disrupts selenoprotein synthesis" J Clin Invest. 126, 992-996.

  • Tani Y, Kimura K,  Mihara H. (2016) "Purification and properties of 4-methyl-5-hydroxyethylthiazole kinase from Escherichia coli" Biosci. Biotechnol. Biochem.  80/3, 514-517.

2015

  • Equar MY, Tani Y, Mihara H. (2015) "Purification and properties of glycine oxidase from Pseudomonas putida KT2440" J. Nutr. Sci. Vitaminol. 61, 506-510.

  • Tani Y, Miyake R, Yukami R, Dekishima Y, China H, Saito S, Kawabata H, Mihara H. (2015) "Functional expression of L-lysine alpha-oxidase from Scomber japonicus in Escherichia coli for one-pot synthesis of L-pipecolic acid from DL-lysine" Appl. Microbiol. Biotechnol. 99, 5045-5054.

  • Tani Y, Omatsu K, Saito S, Miyake R, Kawabata H, Ueda M, Mihara H. (2015) "Heterologous expression of L-lysine alpha-oxidase from Scomber japonicus in Pichia pastoris and functional characterization of the recombinant enzyme" J Biochem. 157, 201-210.

  • Tobe R, Carlson BA, Tsuji PA, Lee BJ, Gladyshev VN, Hatfield DL. (2015) "Differences in redox regulatory systems in human lung and liver tumors suggest different avenues for therapy authors" Cancers. 10, 2262-2276.

  • Bang J, Huh JH, Na JW, Lu Q, Carlson BA, Tobe R, Tsuji PA, Gladyshev VN, Hatfield DL, Lee BJ. (2015) "Cell Proliferation and motility are inhibited by G1 phase arrest in 15-kDa selenoprotein-deficient chang liver cells" Mol Cells. 38, 457-65.

  • Bang J, Jang M, Huh JH, Na JW, Shim M, Carlson BA, Tobe R, Tsuji PA, Gladyshev VN, Hatfield DL, Lee BJ. (2015) "Deficiency of the 15-kDa selenoprotein led to cytoskeleton remodeling and non-apoptotic membrane blebbing through a RhoA/ROCK pathway" Biochem Biophys Res Commun. 456, 884-890.

  • Carlson BA, Tobe R, Tsuji PA, Yoo M-H, Feigenbaum L, Tessarollo L, Lee BJ, Schweizer U, Gladyshev VN, Hatfield D.L. (2015) "Diversity of selenium functions in health and disease" CRC Press, London, UK. Chapter 4.

2014

  • Equar MY, Tani Y, Mihara H. (2015) "Purification and properties of glycine oxidase from Pseudomonas putida KT2440" J. Nutr. Sci. Vitaminol. 61, 506-510.

  • Tani Y, Miyake R, Yukami R, Dekishima Y, China H, Saito S, Kawabata H, Mihara H. (2015) "Functional expression of L-lysine alpha-oxidase from Scomber japonicus in Escherichia coli for one-pot synthesis of L-pipecolic acid from DL-lysine" Appl. Microbiol. Biotechnol. 99, 5045-5054.

  • Tani Y, Omatsu K, Saito S, Miyake R, Kawabata H, Ueda M, Mihara H. (2015) "Heterologous expression of L-lysine alpha-oxidase from Scomber japonicus in Pichia pastoris and functional characterization of the recombinant enzyme" J Biochem. 157, 201-210.

  • Tobe R, Carlson BA, Tsuji PA, Lee BJ, Gladyshev VN, Hatfield DL. (2015) "Differences in redox regulatory systems in human lung and liver tumors suggest different avenues for therapy authors" Cancers. 10, 2262-2276.

  • Bang J, Huh JH, Na JW, Lu Q, Carlson BA, Tobe R, Tsuji PA, Gladyshev VN, Hatfield DL, Lee BJ. (2015) "Cell Proliferation and motility are inhibited by G1 phase arrest in 15-kDa selenoprotein-deficient chang liver cells" Mol Cells. 38, 457-65.

  • Bang J, Jang M, Huh JH, Na JW, Shim M, Carlson BA, Tobe R, Tsuji PA, Gladyshev VN, Hatfield DL, Lee BJ. (2015) "Deficiency of the 15-kDa selenoprotein led to cytoskeleton remodeling and non-apoptotic membrane blebbing through a RhoA/ROCK pathway" Biochem Biophys Res Commun. 456, 884-890.

  • Carlson BA, Tobe R, Tsuji PA, Yoo M-H, Feigenbaum L, Tessarollo L, Lee BJ, Schweizer U, Gladyshev VN, Hatfield D.L. (2015) "Diversity of selenium functions in health and disease" CRC Press, London, UK. Chapter 4.

~2013

  • Tani Y, Nakamura K, Sawa R, Nishio M, Saito S, Ito M, Itonori S, Mihara H. (2013) "Novel neogala-series glycosphingolipids with a terminal glucose residue from the fungus Mariannaea elegans" Biosci Biotech Biochem. 77, 754-759.

  • Hidese R, Mihara H, Kurihara T, Esaki N. (2012) "Pseudomonas putida PydR, a RutR-like transcriptional regulator, represses the dihydropyrimidine dehydrogenase gene in the pyrimidine reductive catabolic pathway" J Biochem. 152, 341-6.

  • Mihara H, Esaki N. (2012) "Selenocysteine Lyase: Mechanism, Structure, and Biological Role" Selenium: Its Molecular Biology and Role in Human and Health (Hatfield DL, Berry MJ, Gladyshev VN. eds.), Springer, New York. pp 95-105.

  • Saito S, Yamano K, Ueda H, Tani Y, Hayakawa M, Tsuji A, Mihara H. (2012) "Effects of a supernatant from Aspergillus oryzae culture on the regulation of crassulacean acid metabolism (CAM) and pinitol biosynthesis in the common ice plant, Mesembryanthemum crystallinum L." Trace Nutr Res. 29, 51-57.

  • Mihara H. (2012) 『セレンディピティ』 バイオサイエンスとインダストリー. 70, 144.

  • Mihara H. (2011) "Structure and function of selenocysteine lyase and cysteine desulfurase" Amino Acids. 41, S77-S78.

  • Tamura, T., Sato, K., Komori, K., Imai, T., Kuwahara, M., Okugochi, T., Mihara, H., Esaki, N. & Inagaki, K. (2011) Selenite reduction by the thioredoxin system: kinetics and identification of protein-bound selenide, Biosci Biotechnol Biochem. 75, 1184-7.

  • Omori, T., Honda, A., Mihara, H., Kurihara, T. & Esaki, N. (2011) Identification of novel mammalian phospholipids containing threonine, aspartate, and glutamate as the base moiety, J Chromat B. 879, 3296-3302.

  • Mihara, H. (2011) 鉄硫黄クラスター・セレンタンパク質生合成を司る酵素, 生化学. 83, 1003-1015.

  • Mihara, H. (2011) 硫黄・セレン供給酵素群による含硫・含セレンバイオファクターの生成機構, バイオサイエンスとインダストリー. 69, 471-475.

  • Kurokawa, S., Takehashi, M., Tanaka, H., Mihara, H., Kurihara, T., Tanaka, S., Hill, K., Burk, R. & Esaki, N. (2011) Mammalian selenocysteine lyase is involved in selenoprotein biosynthesis, J Nutr Sci Vitaminol. 57, 298-305.

  • Kawamoto, J., Sato, T., Nakasone, K., Kato, C., Mihara, H., Esaki, N. & Kurihara, T. (2011) Favourable effects of eicosapentaenoic acid on the late step of the cell division in a piezophilic bacterium, Shewanella violacea DSS12, at high-hydrostatic pressures, Environ Microbiol. 13, 2293-2298.

  • Hidese, R., Mihara, H., Kurihara, T. & Esaki, N. (2011) Escherichia coli dihydropyrimidine dehydrogenase is a novel NAD-dependent heterotetramer essential for the production of 5,6-dihydrouracil, J Bacteriol. 193, 989-93.

  • Hidese, R., Mihara, H. & Esaki, N. (2011) Bacterial cysteine desulfurase: versatile key players in biosynthetic pathways of sulfur-containing biofactors, Appl Microbiol Biot. 91, 47-61.

  • Zhang, W. J., Urban, A., Mihara, H., Leimkuhler, S., Kurihara, T. & Esaki, N. (2010) IscS Functions as a Primary Sulfur-donating Enzyme by Interacting Specifically with MoeB and MoaD in the Biosynthesis of Molybdopterin in Escherichia coli, J Biol Chem. 285, 2302-2308.

  • Yoshimura, T., Mihara, H., Ohshima, T. & Tanizawa, K. (2010) Kenji Soda--researching enzymes with the spirit of an alpinist, J Biochem. 148, 371-9.

  • Tobe, R., Mihara, H., Kurihara, T. & Esaki, N. (2010) Specific transfer of selenium in selenoprotein biosynthesis, Biomed Res Trace Elem. 21, 187-193.

  • Omori, T., Mihara, H., Kurihara, T. & Esaki, N. (2010) Distribution of phosphatidyl-D-serine in rat, Biosci Biotechnol Biochem. 74, 1953-1955.

  • Omi, R., Kurokawa, S., Mihara, H., Hayashi, H., Goto, M., Miyahara, I., Kurihara, T., Hirotsu, K. & Esaki, N. (2010) Reaction mechanism and molecular basis for selenium/sulfur discrimination of selenocysteine lyase, J Biol Chem. 285, 12133-9.

  • Kotera, M., Bayashi, T., Hattori, M., Tokimatsu, T., Goto, S., Mihara, H. & Kanehisa, M. (2010) Comprehensive genomic analysis of sulfur-relay pathway genes, Genome Inform. 24, 104-15.

  • Mihara, H. & Esaki, N. (2010) Synthesis of L-Pipecolic Acid with D1-Piperidine-2-carboxylate Reductase from Pseudomonas putida in Practical Methods for Biocatalysis and Biotransformations (Whittall, J. & Sutton, P. W., eds) pp. 310-313, Wiley, Chichester, UK.

  • Yasuda, M. H., Ueda, M., Okano, K., Mihara, H. & Esaki, N. (2009) Enzymatic Synthesis of Unnatural Amino Acids in Asymmetric Synthesis and Application of α-Amino Acids (Soloshonok, V. A. & Izawa, K., eds), ACS Publications, Washington, DC.

  • Yamauchi, T., Goto, M., Wu, H. Y., Uo, T., Yoshimura, T., Mihara, H., Kurihara, T., Miyahara, I., Hirotsu, K. & Esaki, N. (2009) Serine racemase with catalytically active lysinoalanyl residue, J Biochem. 145, 421-4.

  • Tobe, R., Mihara, H., Kurihara, T. & Esaki, N. (2009) Identification of proteins interacting with selenocysteine lyase, Biosci Biotechnol Biochem. 73, 1230-2.

  • Omori, T., Mihara, H., Kurihara, T. & Esaki, N. (2009) Occurrence of phosphatidyl-D-serine in the rat cerebrum, Biochem Biophys Res Commun. 382, 415-8.

  • Mihara, H. (2009) Discrimination between selenium and sulfur by enzymes [Japanese], Biomed Res Trace Elem. 20, 218-225.

  • Kawamoto, J., Kurihara, T., Yamamoto, K., Nagayasu, M., Tani, Y., Mihara, H., Hosokawa, M., Baba, T., Sato, S. B. & Esaki, N. (2009) Eicosapentaenoic acid plays a beneficial role in membrane organization and cell division of a cold-adapted bacterium, Shewanella livingstonensis Ac10, J Bacteriol. 191, 632-40.

  • Jitsumori, K., Omi, R., Kurihara, T., Kurata, A., Mihara, H., Miyahara, I., Hirotsu, K. & Esaki, N. (2009) X-Ray Crystallographic and Mutational Studies of Fluoroacetate Dehalogenase from Burkholderia sp Strain FA1, J Bacteriol. 191, 2630-2637.

  • Imai, T., Mihara, H., Kurihara, T. & Esaki, N. (2009) Selenocysteine is selectively taken up by red blood cells, Biosci Biotechnol Biochem. 73, 2746-8.

  • Imai, T., Mihara, H., Kurihara, T. & Esaki, N. (2009) Possible Role of Red Blood Cells in Selenocysteine Metabolism Trace Nutri Res. 26, 22-25.

  • Hidese R, Mihara H, Esaki N. (2009) 『大腸菌由来新規 NADH 依存性ジヒドロピリミジンデヒドロゲナーゼの機能解析』ビタミン. 83, 528-532.

  • Goto, M., Yamauchi, T., Kamiya, N., Miyahara, I., Yoshimura, T., Mihara, H., Kurihara, T., Hirotsu, K. & Esaki, N. (2009) Crystal Structure of a Homolog of Mammalian Serine Racemase from Schizosaccharomyces pombe, J Biol Chem. 284, 25944-25952.

  • Zhang W, Mihara H, Kurihara T, Esaki N. (2008) 『モリブドプテリン生合成におけるシステインデスルフラーゼ IscS の役割』ビタミン. 82, 645-650.

  • Zhang, W., Mihara, H., Kurihara, T. & Esaki, N. (2008) Investigation of the Roles of Cysteine Desulfurases in the Molybdopterin Synthesis in Escherichia coli, Trace Nutri Res. 25, 152-157

  • Takahata, M., Tamura, T., Abe, K., Mihara, H., Kurokawa, S., Yamamoto, Y., Nakano, R., Esaki, N. & Inagaki, K. (2008) Selenite Assimilation into Formate Dehydrogenase H Depends on Thioredoxin Reductase in Escherichia coli, J Biochem. 143, 467-473.

  • Muramatsu, H., Mihara, H., Kurihara, T. & Esaki, N. (2008) N-アルキルアミノ酸と環状イミノ酸の不斉合成に利用できる酵素, ファインケミカル. 37, 12-21.

  • Mihara H, Hidese R, Yamane M, Kurihara T, Esaki N. (2008) "The iscS gene deficiency affects the expression of pyrimidine metabolism genes" Biochem Biophys Res Commun. 372, 407-411.

  • Kurokawa, S., Mihara, H., Yokoyama, I., Mochizuki, M., Yodoi, J., Tamura, T., Kurihara, T. & Esaki, N. (2008) Thioredoxin Reductase 1 Is Important for Selenoprotein Biosynthesis in HeLa Cells, Biomed Res Trace Elem. 19, 84-87.

  • Esaki, N. & Mihara, H. (2008) Function and biosynthesis of selenoproteins [Japanese], Biomed Res Trace Elem. 19, 308-316.

  • Abe, K., Mihara, H., Tobe, R. & Esaki, N. (2008) Characterization of human selenocysteine synthase involved in selenoprotein biosynthesis, Biomed Res Trace Elem. 19, 80-83.

  • Abe, K., Mihara, H., Nishijima, Y., Kurokawa, S. & Esaki, N. (2008) Functional analysis of two homologous mouse selenophosphate synthetases, Biomed Res Trace Elem. 19, 76-79.

  • Yoshida, M., Oikawa, T., Obata, H., Abe, K., Mihara, H. & Esaki, N. (2007) Biochemical and genetic analysis of the γ-resorcylate (2,6-dihydroxybenzoate) catabolic pathway in Rhizobium sp strain MTP-10005: Identification and functional analysis of its gene cluster, J Bacteriol. 189, 1573-1581.

  • Yamanishi, Y., Mihara, H., Osaki, M., Muramatsu, H., Esaki, N., Sato, T., Hizukuri, Y., Goto, S. & Kanehisa, M. (2007) Prediction of missing enzyme genes in a bacterial metabolic network - Reconstruction of the lysine-degradation pathway of Pseudomonas aeruginosa, FEBS J. 274, 2262-2273.

  • Mihara H, Esaki N. (2007) 『シュードモナスのリジン分解経路におけるミッシング酵素の同定と解析』ビタミン. 81, 595-600.

  • Mihara H, Esaki N. (2007) 『Selenoproteins [セレンタンパク質]』 Kinzoku [金属]. 77, 262-268.

  • Fujita, M., Mihara, H., Goto, S., Esaki, N. & Kanehisa, M. (2007) Mining prokaryotic genomes for unknown amino acids: a stop-codon-based approach, BMC Bioinformatics. 8, 225.

  • Yasuda, M., Ueda, M., Muramatsu, H., Mihara, H. & Esaki, N. (2006) Enzymatic synthesis of cyclic amino acids by N-methyl-L-amino acid dehydrogenase from Pseudomonas putida, Tetrahedron Asymmet. 17, 1775-1779.

  • Muramatsu, H., Mihara, H., Yasuda, M., Ueda, M., Kurihara, T. & Esaki, N. (2006) Enzymatic synthesis of L-pipecolic acid by Δ1-piperideine-2-carboxylate reductase from Pseudomonas putida, Biosci Biotechnol Biochem. 70, 2296-2298.

  • Mihara, H., Kurokawa, S., Omi, R., Kurihara, T., Hirotsu, K. & Esaki, N. (2006) Selenoprotein biosynthesis and selenium-specific enzymes, Biomed Res Trace Elem. 17, 355-359.

  • Mihara H, Esaki N. (2006) 『セレン化合物とピリドキサール5´-リン酸酵素の生化学』 ビタミン. 80, 587-595.

  • Mihara H,  Esaki N. (2006)『代謝経路データベース上のミッシング酵素の同定 -Identification of missing enzymes in metabolic pathway databases-』 バイオサイエンスとインダストリー. 64, 553-557.

  • Mihara H,  Esaki N. (2006) 『遺伝子と微量元素』 治療. 88, 1859-1864.

  • Mihara H. (2006) 『N-メチル-L-アミノ酸とL-ピペコリン酸の合成酵素 -An enzyme synthesizing N-methyl-L-amino acid and pipecolic acid-』バイオサイエンスとインダストリー. 64, 31-32.

  • Muramatsu, H., Mihara, H., Kakutani, R., Yasuda, M., Ueda, M., Kurihara, T. & Esaki, N. (2005) The putative malate/lactate dehydrogenase from Pseudomonas putida is an NADPH-dependent Δ1-piperideine-2-carboxylate/Δ1-pyrroline-2-carboxylate reductase involved in the catabolism of D-lysine and D-proline, J Biol Chem. 280, 5329-5335.

  • Muramatsu, H., Mihara, H., Goto, M., Miyahara, I., Hirotsu, K., Kurihara, T. & Esaki, N. (2005) A new family of NAD(P)H-dependent oxidoreductases distinct from conventional Rossmann-fold proteins, J Biosci Bioeng. 99, 541-547.

  • Mihara, H., Muramatsu, H., Kakutani, R., Yasuda, M., Ueda, M., Kurihara, T. & Esaki, N. (2005) N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida - A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily, FEBS J. 272, 1117-1123.

  • Kurokawa, S., Mihara, H., Takehashi, M., Tanaka, S., Kurihara, T. & Esaki, N. (2005) RNAi-mediated knock down of mammalian selenocysteine lyase, Biomed Res Trace Elem. 16, 321-324.

  • Goto, M., Muramatsu, H., Mihara, H., Kurihara, T., Esaki, N., Omi, R., Miyahara, I. & Hirotsu, K. (2005) Crystal structures of Δ1-piperideine-2-carboxylate/Δ1-pyrroline-2-carboxylate reductase belonging to a new family of NAD(P)H-dependent oxidoreductases - Conformational change, substrate recognition, and stereochemistry of the reaction, J Biol Chem. 280, 40875-40884.

  • Esaki, N., Mihara, H., Hara, M. & Ueda, M. (2005) Manufacture of optically active cyclic amino acids with N-methyl-L-amino acid dehydrogenase in Jpn Kokai Tokkyo Koho (Koho, J. K. T., ed)Japan.

  • Abe, K., Ebata, I., Kazuoka, T., Mihara, H., Kurihara, T. & Esaki, N. (2005) Protein interaction between selenophosphate synthetase and IscS, Biomed Res Trace Elem. 16, 325-327.

  • Muramatsu, H., Mihara, H., Kakutani, R., Yasuda, M., Ueda, M., Kurihara, T. & Esaki, N. (2004) Enzymatic synthesis of N-methyl-L-phenylalanine by a novel enzyme, N-methyl-L-amino acid dehydrogenase, from Pseudomonas putida, Tetrahedron Asymmet. 15, 2841-2843.

  • Kurokawa, S., Mihara, H., Kurihara, T. & Esaki, N. (2004) Expression analysis of mammalian selenocysteine lyase, Biomed Res Trace Elem. 15, 278-280.

  • Pilon-Smits, E. A. H., Garifullina, G. F., Abdel-Ghany, S., Kato, S. I., Mihara, H., Hale, K. L., Burkhead, J. L., Esaki, N., Kurihara, T. & Pilon, M. (2003) Characterization of a NifS-like chloroplast protein from Arabidopsis. Implications for its role in sulfur and selenium metabolism (vol 130, pg 1309, 2002), Plant Physiol. 131, 379-379.

  • Pilon, M., Owen, J. D., Garifullina, G. F., Kurihara, T., Mihara, H., Esaki, N. & Pilon-Smits, E. A. H. (2003) Enhanced selenium tolerance and accumulation in transgenic Arabidopsis expressing a mouse selenocysteine lyase, Plant Physiol. 131, 1250-1257.

  • Mihara, H. (2003) まだまだ謎の多いビオチン生合成, 生物工学会誌. 81, 164.

  • Kwak, M. S., Mihara, H. & Esaki, N. (2003) A novel regulatory function of selenocysteine lyase, a unique catalyst to modulate major urinary protein, J Mol Catal B-Enzym. 23, 367-372.

  • Kuwana, E., Kurokawa, S., Mihara, H., Kurihara, T., Yoshimura, T. & Esaki, N. (2003) Effects of selenium deficiency on the expression of selenoprotein mRNAs in mouse brain, Biomed Res Trace Elem. 14, 293-296.

  • Kurihara, T., Mihara, H., Kato, S., Yoshimura, T. & Esaki, N. (2003) Assembly of iron-sulfur clusters mediated by cysteine desulfarases, IscS, CsdB and CSD, from Escherichia coli, Biochim Biophys Acta. 1647, 303-309.

  • Esaki, N., Mihara, H., Hara, M. & Ueda, M. (2003) Novel Pseudomonas putida dehydrogenase catalyzing synthesis of N-alkyl amino acid from pyruvate and alkylamine, encoding gene, and biosynthetic uses. in WO2003072770

  • Esaki, N. & Mihara, H. (2003) 鉄硫黄クラスター形成にかかわるピリドキサル酵素の機能、構造、触媒機構, ビタミン. 77, 285-295.

  • Tokumoto, U., Nomura, S., Minami, Y., Mihara, H., Kato, S., Kurihara, T., Esaki, N., Kanazawa, H., Matsubara, H. & Takahashi, Y. (2002) Network of protein-protein interactions among iron-sulfur cluster assembly proteins in Escherichia coli, J Biochem. 131, 713-719.

  • Pilon-Smits, E. A. H., Garifullina, G. F., Abdel-Ghany, S., Kato, S. I., Mihara, H., Hale, K. L., Burkhead, J. L., Esaki, N., Kurihara, T. & Pilon, M. (2002) Characterization of a NifS-Like chloroplast protein from Arabidopsis. Implications for its role in sulfur and selenium metabolism, Plant Physiol. 130, 1309-1318.

  • Mihara, H., Kato, S., Lacourciere, G. M., Stadtman, T. C., Kurihara, T., Tokumoto, U., Takahashi, Y. & Esaki, N. (2002). Role of NifS homologs in selenium metabolism. Paper presented at the The 19th Symposium on Trace Nutrients Research.

  • Mihara, H., Kato, S., Lacourciere, G. M., Stadtman, T. C., Kennedy, R. A. J. D., Kurihara, T., Tokumoto, U., Takahashi, Y. & Esaki, N. (2002) The iscS gene is essential for the biosynthesis of 2-selenouridine in tRNA and the selenocysteine-containing formate dehydrogenase H, P Natl Acad Sci USA. 99, 6679-6683.

  • Mihara, H., Fujii, T., Kato, S., Kurihara, T., Hata, Y. & Esaki, N. (2002) Structure of external aldimine of Escherichia coli CsdB, an IscS/NifS homolog: Implications for its specificity toward selenocysteine, J Biochem. 131, 679-685.

  • Mihara, H. & Esaki, N. (2002) セレンの生化学, ビタミン. 76, 7-9.

  • Mihara, H. & Esaki, N. (2002) Selenocysteine lyase from mouse liver, Methods Enzymol. 347, 198-203.

  • Mihara, H. & Esaki, N. (2002) Bacterial cysteine desulfurases: their function and mechanisms, Appl Microbiol Biot. 60, 12-23.

  • Kwak, M. S., Mihara, H. & Esaki, N. (2002) Protein Interaction between Selenocysteine Lyase and MUP-I, Biomed Res Trace Elem. 13, 238-239.

  • Kato, S., Mihara, H., Kurihara, T., Takahashi, Y., Tokumoto, U., Yoshimura, T. & Esaki, N. (2002) Cys-328 of IscS and Cys-63 of IscU are the sites of disulfide bridge formation in a covalently bound IscS/IscU complex: Implications for the mechanism of iron-sulfur cluster assembly, P Natl Acad Sci USA. 99, 5948-5952.

  • Mihara, H., Kato, S., Kurihara, T., Yoshimura, T., Esaki, N., Lacourciere, G. M. & Stadtman, T. C. (2001) Role of Cysteine Desulfurase in the Biosynthesis of Formate Dehydrogenase H Containing Mo, Molybdopterin, Selenocysteine, and Fe4S4 Cluster, Biomed Res Trace Elem. 12, 261-262.

  • Kato, S., Mihara, H., Kurihara, T., Yoshimura, T. & Esaki, N. (2001). Screening and Identification of a Protein Interacting with IscU. Paper presented at the The 18th Symposium on Trace Nutrients Research.

  • Kashiwa, M., Ike, M., Mihara, H., Esaki, N. & Fujita, M. (2001) Removal of soluble selenium by a selenate-reducing bacterium Bacillus sp. SF-1, Biofactors. 14, 261-265.

  • Mihara, H., Kurihara, T., Yoshimura, T. & Esaki, N. (2000) Kinetic and mutational studies of three NifS homologs from Escherichia coli: Mechanistic difference between L-cysteine desulfurase and L-selenocysteine lyase reaction, J Biochem. 127, 559-567.

  • Mihara, H., Kurihara, T., Watanabe, T., Yoshimura, T. & Esaki, N. (2000) cDNA cloning, purification, and characterization of mouse liver selenocysteine lyase - Candidate for selenium delivery protein in selenoprotein synthesis, J Biol Chem. 275, 6195-6200.

  • Lacourciere, G. M., Mihara, H., Kurihara, T., Esaki, N. & Stadtman, T. C. (2000) Escherichia coli NifS-like proteins provide selenium in the pathway for the biosynthesis of selenophosphate, J Biol Chem. 275, 23769-23773.

  • Kato, S., Mihara, H., Kurihara, T., Yoshimura, T. & Esaki, N. (2000). A NifS homolog operating the biogenesis of elemental sulfur and elemental selemium: Analysis of their ability to form iron-sulfur cluster [元素状硫黄と元素状セレンのバイオジェネシスを司るNifS相同タンパク質： 鉄-硫黄クラスター形成能の解明]. Paper presented at the The 17th Symposium on Trace Nutrients Research.

  • Kato, S., Mihara, H., Kurihara, T., Yoshimura, T. & Esaki, N. (2000) Gene cloning, purification, and characterization of two cyanobacterial NifS homologs driving iron-sulfur cluster formation, Biosci Biotechnol Biochem. 64, 2412-2419.

  • Fujii, T., Maeda, M., Mihara, H., Kurihara, T., Esaki, N. & Hata, Y. (2000) Structure of a NifS homologue: X-ray structure analysis of CsdB, an Escherichia coli counterpart of mammalian selenocysteine lyase, Biochemistry. 39, 1263-1273.

  • Mihara, H., Maeda, M., Fujii, T., Kurihara, T., Hata, Y. & Esaki, N. (1999) A nifS-like gene, csdB, encodes an Escherichia coli counterpart of mammalian selenocysteine lyase - Gene cloning, purification, characterization and preliminary x-ray crystallographic studies, J Biol Chem. 274, 14768-14772.

  • Mihara, H. (1999) Enzymological studies of cysteine desulfurase and selenocysteine lyase, Kyoto University.

  • Esaki, N., Mihara, H., Kurihara, T., Yoshimura, T. & Soda, K. (1999). Structure and function of an E. coli NifS homolog with selenocysteine lyase activity [セレノシステインリアーゼ活性を持つ大腸菌NifSホモログの構造と機能]. Paper presented at the The 16th Symposium on Trace Nutrients Research.

  • Mihara H, Kurihara T, Yoshimura T, Soda K, Esaki N. (1997) "Cysteine sulfinate desulfinase, a NIFS-like protein of Escherichia coli with selenocysteine lyase and cysteine desulfurase activities - Gene cloning, purification, and characterization of a novel pyridoxal enzyme" J Biol Chem. 272, 22417-22424.

  • Mihara H. (1995) 『セレンによるAIDS治療の可能性』 ビタミン. 69, 226-228.

  • Watanabe K, Masuda T, Ohashi H, Mihara H, Suzuki Y. (1994) "Multiple proline substitutions cumulatively thermostabilize Bacillus cereus ATCC7064 oligo-1,6-glucosidase - irrefragable proof supporting the proline rule" Eur J Biochem. 226, 277-283.

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